Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

Development of tag-probe system for analysis of protein dynamics in cells

Fluorescent imaging by ZIP tag-probe system can be conducted without washing steps because of the use of solvatchromic dyes. It has been shown that transiently expressed tag-fused proteins can be monitored in cells. In this project, we combine ZIP tag-probe system and genome editing to analyze dynamics of endogenous expression of target proteins in cells.