Bioluminescence imaging (BLI) is a powerful technique for visualizing biological processes in living animals. Although BLI achives extremly high signal to noise ratio in noninvasive animal imaging, spatial resolution of this method is poor due to light scattering in living tissues. In addition, a series of bioluminescence tomography (BLT) tecniques fails to provide sufficient spatial resolution in animal imaging. These facts emphasize the needs to explore new apporaches alternative to current BLT based on optical diffusion equations and tissue optical parameters. In this project, we propose to develop a unique genetically-encoded reporter system to improve spatial resolution of BLT.