Gifu Pharmaceutical University

Development of nano-imaging methods of oxidative stress by anchoring on cytoskeleton

The conventional live imaging probes can detect target biomolecules at each organelle, but it has been challenging to observe the target molecules at fixed points in the whole cell thoroughly. In this research, our group is going to develop new methods to observe reactive oxygen species (ROS) without being affected by free intracellular diffusion. The main strategy is based on fixation of the molecular probes of ROS onto cytoskeletons which exist uniformly in the intracellular space. Our purpose ranges over the development of nano-imaging methods which are useful to super-resolution fluorescence microscopic imaging and electron microscopic imaging of intracellular ROS production.