In this study, we address evaluation of cell-to-cell variability, safety, spatial distribution, conformational changes and intracellular trafficking of our vectors, and visualization of oxidative stress using tissue optical clearing and fluorescence to accelerate the development of safe and effective in vivo gene transfer. We utilize novel tissue optical clearing method ‘Seebest’ without detergent for the purpose.
2018
Wang S., Fumoto S., Miyamoto H., Tanaka M., Nishida K. (2018) Edaravone, a cytoprotective drug, enhances transgene expression mediated by lipoplexes in HepG2 cells and mice. International Journal of Pharmaceutics, 548: 173-181.
doi: 10.1016/j.ijpharm.2018.06.068.
2017
Nishimura K, Fumoto S, Fuchigami Y, Hagimori M, Maruyama K, Kawakami S.(2017) Effective Intraperitoneal Gene Transfection System Using Nanobubbles and Ultrasound Irradiation. Drug Delivery, 24 (1), 737-744.
doi: 10.1080/10717544.2017.1319433.
麓伸太郎 (2017) ＤＤＳ先端技術の製剤への応用開発 第4章第9節 「非ウイルスベクターによる遺伝子導入技術の開発」, 技術情報協会, 221-232.
2016
Fumoto S., Nishimura K., Nishida K., Kawakami S. (2016) Three-Dimensional Imaging of the Intracellular Fate of Plasmid DNA and Transgene Expression: ZsGreen1 and Tissue Clearing Method CUBIC are an Optimal Combination for Multicolor Deep Imaging in Murine Tissues. PLoS One, 11: e0148233.
doi:10.1371/journal.pone.0148233.
Fumoto S., Taniguchi N., Ikai Y., Yoshikawa N., Miyamoto H., Sasaki H., Hashida M., Kawakami S., Nishida K. (2016) The Insertion Timing of PEGylated Lipids to Galactosylated Lipoplexes Is Important for Liver-Selective Transfection in Mice. Gene and Cell Therapy, 1: 1-11.
doi: 10.11648/j.ijgg.20160406.15.
