The purpose of this work is realization of highly penetrative, high resolution, in vivo, real-time imaging. First, we will develop highly functional ultrashort pulse fiber lasers. Then, using the developed light source, we will develop multi-functional imaging system with the combination of highly penetrateive, high resolution optical coherence tomography (OCT)/ optical coherence microscopy (OCM) and multi-photon microscopy (MPM) at longer wavelength range. Especially, we will develop electrically controlled, high power, rapid wavelength tunable ultrashort pulse source, and we will realize automatically controlled high resolution OCT/OCM and MPM. Besides, we also aim to develop novel imaging technique via the collaboration with other teams by use of our light source technologies.