The cell fate within various tissues is determined by cell-cell adhesion and paracrine factors. To understand such cell-cell signaling in live tissues, we need to visualize the intercellular communication in the absence and presence of various perturbations. In this project, we will improve our techniques to visualize the activities of protein kinases and small GTPases in living mice, so that we can detect the signaling activities with higher sensitivity and in near-infrared wavelength fluorescence. We will also develop chemical compounds that could modulate the activities of intracellular signaling molecules. With this approaches combined, we will shed new light to the regulatory mechanism of cellular response and cell fate.
2020
Ito H, Tsunoda T, Riku M, Inaguma S, Inoko A, Murakami H, Ikeda H, Matsuda M, Kasai K. (2020) Indispensable role of STIL in the regulation of cancer cell motility through the lamellipodial accumulation of ARHGEF7-PAK1 complex. Oncogene, 39 (9): 1931-1943.
doi: 10.1038/s41388-019-1115-9.
2019
Komatsubara AT, Goto Y, Kondo Y, Matsuda M, Aoki K. (2019) Single-cell quantification of the concentrations and dissociation constants of endogenous proteins. J. Biol. Chem., 294 (15): 6062-6072.
doi: 10.1074/jbc.RA119.007685.
Kinjo T., Terai K., Horita S., Nomura N., Sumiyama K., Togashi K., Iwata S. Matsuda M. (2019) FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics, Nature Methods, 16 (10): 1029-1036.
doi: 10.1038/s41592-019-0541-5.
Muta Y., Matsuda M., Imajo M. (2019) Divergent Dynamics and Functions of ERK MAP Kinase Signaling in Development, Homeostasis and Cancer: Lessons from Fluorescent Bioimaging. Cancers (Basel) ,11(4).
doi: 10.3390/cancers11040513.
Terai K., Imanishi A., Li C., Matsuda M. (2019) Two Decades of Genetically Encoded Biosensors Based on Forster Resonance Energy Transfer, Cell Struct. Funct. 44 (2): 153-169.
doi: 10.1247/csf.18035.
2018
Konishi Y., Terai K., Furuta Y., Kiyonari H., Abe T., Ueda Y., Kinashi T., Hamazaki Y., Takaori-Kondo A., Matsuda M. (2018) Live-Cell FRET Imaging Reveals a Role of Extracellular Signal-Regulated Kinase Activity Dynamics in Thymocyte Motility. iScience, 10: 98-113.
doi:10.1016/j.isci.2018.11.025.
Sano T., Kobayashi T., Ogawa O., Matsuda M. (2018) Gliding Basal Cell Migration of the Urothelium during Wound Healing. Am. J. Pathol., 188: 2564-2573.
doi:10.1016/j.ajpath.2018.07.010.
Muta Y., Matsuda M., Imajo M. (2018) Dynamic ERK signaling regulation in intestinal tumorigenesis. Molecular & Cellular Oncology, 5: e1506684.
doi:10.1080/23723556.2018.1506684.
Miura H., Kondo Y., Matsuda M., Aoki K. (2018) Cell-to-Cell Heterogeneity in p38-Mediated Cross-Inhibition of JNK Causes Stochastic Cell Death. Cell Rep., 24: 2658-2668.
doi:10.1016/j.celrep.2018.08.020.
Imanishi A., Murata T., Sato M., Hotta K., Imayoshi I., Matsuda M., Terai K. (2018) A novel morphological marker for the analysis of molecular activities at the single-cell level. Cell Struct. Funct., 43: 129-140.
doi:10.1247/csf.18013.
Maryu G., Miura H., Uda, Y., A, T. K., Matsuda M., Aoki K. (2018) Live-cell imaging with genetically encoded protein kinase activity reporters. Cell Struct. Funct., 43: 61-74.
doi:10.1247/csf.18003.
Komatsu N., Terai K., Imanishi A., Kamioka Y., Sumiyama K., Jin T., Okada Y., Nagai T., Matsuda M. (2018) A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging. Scientific Report, 8: 8984.
doi: 10.1038/s41598-018-27174-x.
Muta Y., Fujita Y., Sumiyama K., Sakurai A., Taketo M.M., Chiba T., Seno H., Aoki K., Matsuda M., Imajo M. (2018) Composite regulation of ERK activity dynamics underlying tumour-specific traits in the intestine. Nature Communications, 9: 2174.
doi: 10.1038/s41467-018-04527-8.
2017
Hiratsuka, T., Sano, T., Kato, H., Komatsu, N., Imajo, M., Kamioka, Y., Sumiyama, K., Banno, F., Miyata, T. Matsuda, M. (2017) Live imaging of extracellular signal-regulated kinase and protein kinase A activities during thrombus formation in mice expressing biosensors based on Forster resonance energy transfer. J. Thromb. Haemat. 15 (7): 1487-1499.
doi: 10.1111/jth.13723.
Konagaya Y., Terai K., Hirao Y., Takakura K., Imajo M., Kamioka Y., Sasaoka N., Kakizuka A., Sumiyama K., Asano T., Matsuda M. (2017) A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs. Cell Rep. 21: 2628-2638.
doi:10.1016/j.celrep.2017.10.113.
Aoki K., Kondo Y., Naoki H., Hiratsuka T., Itoh R.E., Matsuda M. (2017) Propagating Wave of ERK Activation Orients Collective Cell Migration. Dev. Cell, 43(3):305-317.e5.
doi: 10.1016/j.devcel.2017.10.016.
Hiratsuka T., Sano T., Kato H., Komatsu N., Imajo M., Kamioka Y., Sumiyama K., Banno F., Miyata T., Matsuda M. (2017) Live imaging of extracellular signal-regulated kinase and protein kinase A activities during thrombus formation in mice expressing biosensors based on Förster resonance energy transfer. J Thromb. Haemat. 15(7):1487-1499.
doi: 10.1111/jth.13723.
2016
Yamauchi F., Kamioka Y., Yano T. Matsuda M. (2016) In vivo FRET imaging of tumor endothelial cells highlights a role of low PKA activity in vascular hyperpermeability. Cancer Res., 76: 5266-5276.
doi:10.1158/0008-5472.can-15-3534.
Sano T., Kobayashi T., Negoro H., Sengiku A., Hiratsuka T., Kamioka Y., Liou L. S., Ogawa O. Matsuda M. (2016) Intravital imaging of mouse urothelium reveals activation of extracellular signal-regulated kinase by stretch-induced intravesical release of ATP. Physiological reports, 4.
doi:10.14814/phy2.13033.
Yamao M., Aoki K., Yukinawa N., Ishii S., Matsuda M., Naoki H. (2016) Two New FRET Imaging Measures: Linearly Proportional to and Highly Contrasting the Fraction of Active Molecules. PLoS One, 11, e0164254.
doi:10.1371/journal.pone.0164254.
Takaoka S., Kamioka Y., Takakura K., Baba A., Shime H., Seya T., Matsuda M. (2016) Live imaging of TAK1 activation in Lewis lung carcinoma 3LL cells implanted into syngeneic mice and treated with polyI:C. Cancer Sci., 107: 644-652.
doi:10.1111/cas.12923.
Sakamoto K., Fukushima Y., Ito K., Matsuda M., Nagata S., Minato N., Hattori M. (2016) Osteopontin in spontaneous germinal centers inhibits apoptotic cell engulfment and promotes anti-nuclear antibody production in Lupus-prone mice. J. Immunol., 197: 2177-2186.
doi:10.4049/jimmunol.1600987.
Okuchi Y., Imajo M., Mizuno R., Kamioka Y., Miyoshi H., Taketo M. M., Nagayama S., Sakai Y. Matsuda M. (2016) Identification of aging-associated gene expression signatures that precede intestinal tumorigenesis. PLoS One 11, e0162300.
doi: 10.1371/journal.pone.0162300.
Mizuno R., Kamioka Y., Sakai Y., Matsuda M. (2016) Visualization of signaling molecules during neutrophil recruitment in transgenic mice expressing FRET biosensors. Methods Mol. Biol., 1422: 149-160.
doi:10.1007/978-1-4939-3603-8_14.
Maryu G., Matsuda M., Aoki K. (2016) Multiplexed fluorescence imaging of ERK and Akt activities and cell-cycle progression. Cell Struct. Funct., 41: 81-92.
doi:10.1247/csf.16007.
Li C., Imanishi A., Komatsu N., Terai K., Amano M., Kaibuchi K., Matsuda M. (2016) A FRET biosensor for ROCK based on a consensus substrate sequence identified by KISS technology. Cell Struct. Funct., 42: 1-13.
doi:10.1247/csf.16016.
Kawabata N., Matsuda M. (2016) Cell Density-Dependent Increase in Tyrosine-Monophosphorylated ERK2 in MDCK Cells Expressing Active Ras or Raf. PLoS One, 11: e0167940.
doi:10.1371/journal.pone.0167940.
Candeias M.M., Hagiwara M., Matsuda M. (2016) Cancer-specific mutations in p53 induce the translation of Delta160p53 promoting tumorigenesis. EMBO reports 17, 1542-1551.
doi:10.15252/embr.201541956.
2015
Yamao M., Naoki H., Kunida K., Aoki K., Matsuda M., Ishii S. (2015) Distinct predictive performance of Rac1 and Cdc42 in cell migration. Sci. Rep., 5: 17527.
doi:10.1038/srep17527.
Yamaguchi T., Goto A., Nakahara I., Yawata S., Hikida T., Matsuda M., Funabiki K., Nakanishi S. (2015) Role of PKA signaling in D2 receptor-expressing neurons in the core of the nucleus accumbens in aversive learning. Proc. Natl. Acad. Sci. U. S. A., 112: 11383-11388.
doi:10.1073/pnas.1514731112.
Wakayama Y., Fukuhara S., Ando K., Matsuda M., Mochizuki N. (2015) Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish. Dev Cell 32: 109-122.
doi:10.1016/j.devcel.2014.11.024.
Kumagai Y., Naoki H., Nakasho E., Kamioka Y., Kiyokawa E., Matsuda M. (2015) Heterogeneity in ERK activity as visualized by in vivo FRET imaging of mammary tumor cells developed in MMTV-Neu mice. Oncogene, 34: 1051-1057.
doi:10.1038/onc.2014.28.
Komatsubara A.T., Matsuda M., Aoki K. (2015) Quantitative analysis of recombination between YFP and CFP genes of FRET biosensors introduced by lentiviral or retroviral gene transfer. Sci. Rep., 5: 13283.
doi:10.1038/srep13283.
Komatsu N., Fujita Y., Matsuda M., Aoki K. (2015) mTORC1 upregulation via ERK-dependent gene expression change confers intrinsic resistance to MEK inhibitors in oncogenic KRas-mutant cancer cells. Oncogene, 34: 5607-5616.
doi:10.1038/onc.2015.16.
Hiratsuka T., Fujita Y., Naoki H., Aoki K., Kamioka Y., Matsuda M. (2015) Intercellular propagation of extracellular signal-regulated kinase activation revealed by in vivo imaging of mouse skin. eLife, 4: e05178.
doi:10.7554/eLife.05178.
Hirata E., Girotti M.R., Viros A., Hooper S., Spencer-Dene B., Matsuda M., Larkin J., Marais R., Sahai E. (2015) Intravital imaging reveals how BRAF inhibition generates drug-tolerant microenvironments with high integrin beta1/FAK signaling. Cancer Cell, 27: 574-588.
doi:10.1016/j.ccell.2015.03.008.
Goto A., Nakahara I., Yamaguchi T., Kamioka Y., Sumiyama K., Matsuda M., Nakanishi S., Funabiki K. (2015) Circuit-dependent striatal PKA and ERK signaling underlies rapid behavioral shift in mating reaction of male mice. Proc. Natl. Acad. Sci. U. S. A., 112: 6718-6723.
doi:10.1073/pnas.1507121112.
