Medical Institute of Bioregulation, Kyushu University

Developing precision genome editing methods based on an allele-specific indel monitor system

Dramatic prograss of CRISPR-Cas9 technology has increased specificity and efficiency of Cas9 on the target genomic loci. However, an allele-selective editing method has not been established as the two alleles share the same sequences. Here we develop the methods by elucidating the mechanism of Cas9 activity on two alleles, which will be investigated using a novel allele-specific indel monitor system (AIMS). The allele-specific editing tool will be widely used for basic researches and clinical applications, especially in gene correction therapy.