Dramatic prograss of CRISPR-Cas9 technology has increased specificity and efficiency of Cas9 on the target genomic loci. However, an allele-selective editing method has not been established as the two alleles share the same sequences. Here we develop the methods by elucidating the mechanism of Cas9 activity on two alleles, which will be investigated using a novel allele-specific indel monitor system (AIMS). The allele-specific editing tool will be widely used for basic researches and clinical applications, especially in gene correction therapy.
2018
Yoshimura A., Adachi N., Matsuno H., Kawamata M., Yoshioka Y., Kikuchi H., Odaka H., Numakawa T., Kunugi H., Ochiya T., Tamai Y. (2018) The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles. Dis. Model. Mech., 11 (1): dmm028779.
doi:10.1242/dmm.028779.
2017
Kawamata M., Katsuda T., Yamada Y., Ochiya T. (2017) In vitro reconstitution of breast cancer heterogeneity with multipotent cancer stem cells using small molecules. Biochem. Biophys. Res. Commun., 482 (4): 750-757.
doi: 10.1016/j.bbrc.2016.11.106.
Kawamata M., Suzuki A. (2017) Cell fate modification toward the hepatic lineage by extrinsic factors. J. Biochem., 162 (1): 11-16.
doi: 10.1093/jb/mvx028.
Katsuda T., Kawamata M., Hagiwara K., Takahashi R.U., Yamamoto Y., Camargo F.D., Ochiya T. (2017) Conversion of terminally committed hepatocytes to culturable bipotent progenitor cells with regenerative capacity. Cell Stem Cell, 20 (1): 41-55.
doi: 10.1016/j.stem.2016.10.007.