To clarify neural circuit structure and function, we should not only to visualize the whole brain structure but also to analyze the neural response to the stimulation. However, this is challenging in plactice because the spatio-temporal resolution and/or field of view are not enough. In this study, we will conduct research on the following two items:
- To establish a basic imaging technique to break down a trade-off relation between the spatio-temporal resolution and field of view.
- To establish patterned photostimulation with 3D subcelluller resolution.
2020
磯部 圭佑 (2020) 広視野多光子照明と光操作 高速多光子イメージング, 生体の科学, 71, 169-173.
doi: 10.11477/mf.2425201149.
2018
Toda K., Isobe K., Namiki K., Kawano H., Miyawaki A., Midorikawa K. (2018) Interferometric Temporal Focusing Microscopy Using Three-Photon Excitation Fluorescence. Biomed. Opt. Express, 9: 1510-1519.
doi:10.1364/BOE.9.001510.
2017
Toda K., Isobe K., Namiki K., Kawano H., Miyawaki A., Midorikawa K. (2017) Temporal Focusing Microscopy Using Three-Photon Excitation Fluorescence with a 92-fs Yb-fiber Chirped Pulse Amplifier. Biomed. Opt. Express, 8: 2796-2806.
doi:10.1364/BOE.8.002796.
Isobe K., Toda K., Song Q., Kannari F., Kawano H., Miyawaki A., Midorikawa K. (2017) Temporal Focusing Microscopy Combined with Three-dimensional Structured Illumination,” Jpn. J. Appl. Phys. 56: 052501.
doi:10.7567/JJAP.56.052501.