To clarify neural circuit structure and function, we should not only to visualize the whole brain structure but also to analyze the neural response to the stimulation. However, this is challenging in plactice because the spatio-temporal resolution and/or field of view are not enough. In this study, we will conduct research on the following two items:
- To establish a basic imaging technique to break down a trade-off relation between the spatio-temporal resolution and field of view.
- To establish patterned photostimulation with 3D subcelluller resolution.