Several optogenetic tools have been developed in recent years, yet few systems are available that employ red light for photoactivation. Phytochrome B (PhyB)-Phytochrome interacting factor (PIF) is a unique system, in which PhyB binds to PIF under red light illumination, while PhyB-PIF complex dissociates upon infra-red light illumination. However, PhyB-PIF system requires phycocyanobilin, which is a chromophore of light harvesting in photosynthetic organisms, and therefore purification and addition of PCB are needed to render cells sensitive to red/infra-red light in this system. To overcome this issue, we succeeded efficient PCB biosynthesis in mammalian cells for developing a fully genetically encoded optogenetic system with red/infra-red light. In this study, we will attempt to manipulate intracellular signaling in space and time by red/infra-red light illumination, and further to control the behavior of C. elegans by regulating intracellular signaling with red/far-red light.
2019
Komatsubara A.T., Goto Y., Kondo Y., Matsuda M., Aoki K. (2019) Single-cell quantification of the concentrations and dissociation constants of endogenous proteins. J. Biol.Chem., 294(15):6062-6072.
doi:10.1074/jbc.RA119.007685.
2018
Miura H., Kondo Y., Matsuda M., Aoki K. (2018) Cell-to-Cell Heterogeneity in p38-Mediated Cross-Inhibition of JNK Causes Stochastic Cell Death. Cell Rep., 24: 2658-2668.
doi:10.1016/j.celrep.2018.08.020.
Maryu G., Miura H., Uda Y., Komatsubara A.T., Matsuda M., Aoki K. (2018) Live-cell imaging with genetically encoded protein kinase activity reporters. Cell Structure and Function, 43: 61-74.
doi: 10.1247/csf.18003.
2017
Uda Y., Goto Y., Oda S., Kohchi T., Matsuda M., Aoki K. (2017) Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling. Proc. Natl. Acad. Sci. U.S.A., 114 (45): 11962-11967.
doi:10.1073/pnas.1707190114.
Aoki K., Kondo Y., Naoki H., Hiratsuka T., Itoh RE., Matsuda M. (2017) Propagating Wave of ERK Activation Orients Collective Cell Migration. Developmental Cell, 43: 305–317.
doi:10.1016/j.devcel.2017.10.016.