Hideji MURAKOSHI | ResonanceBio

Research Subject

In this project we will develop dark fluorescent proteins for deep-tissue imaging. Since our fluorescent proteins have high absorbance of red wavelength (high permeability in tissue), it will enable deep tissue imaging. The application of dark fluorescent protein as an acceptor for 2-photon FLIM-FRET will improve signal-to-noise ratio and facilitate multi-color FLIM-FRET imaging in deep tissue such as brain slice. Furthermore, we will apply dark fluorescent proteins to mice in vivo and carries out dual observation of intracellular signaling proteins and Ca2+, and try to understand the mechanisms of neuronal circuit development during learning and memory formation.

Publication

2017

Murakoshi H., Shin M., Parra-Bueno P., Szatmari E.M., Shibata A.C., Yasuda R. (2017) Kinetics of endogenous CaMKII required for synaptic plasticity revealed by optogenetic kinase inhibitor. Neuron, 94 (1): 37-47.
doi: 10.1016/j.neuron.2017.02.036.

Murakoshi H., Shibata A.C. (2017) ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement. Scientific Reports, 7: 6791.
doi: 10.1038/s41598-017-07002-4.

村越秀治 (2017) 第２章少数で伝える～神経シナプスのシグナル伝達～. 永井健治・冨樫祐一［編］少数性生物学,日本評論社, 9-16.

村越秀治 (2017) 分子間相互作用を検出する2光子蛍光寿命イメージング. 生体の科学, 医学書院, 68 (5) : 400-401.

村越秀治 (2017) クローズアップ実験法光応答性阻害ペプチドの生化学的機能アッセイ. 実験医学, 羊土社, 2765–2770.

2016

Fujiwara TK, Iwasawa K, Kalay Z, Tsunoyama TA, Watanabe Y, Umemura YM, Murakoshi H, Suzuki KG, Nemoto YL, Morone N, and Kusumi A. (2016) Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane.Mol.Biol.Cell, 27: 1101-1119.
doi: 10.1038/s41598-017-07002-4.

Miyamoto A, Wake H, Ishikawa AW, Eto K, Shibata K, Murakoshi H, Koizumi S, Moorhouse AJ, Yoshimura Y, and Nabekura J. (2016) Microglia contact induces synapse formation in developing somatosensory cortex. Nature Communications, 7: 12540.
doi: 10.1038/ncomms12540.

Hedrick NG, Harward SC, Hall CE, Murakoshi H, McNamara JO, and Yasuda R. (2016) Rho GTPase complementation underlies BDNF-dependent homo- and heterosynaptic plasticity. Nature, 538: 104-108.
doi: 10.1038/nature19784.

Phengchat R, Takata H, Morii K, Inada N, Murakoshi H, Uchiyama S, and Fukui K. (2016) Calcium ions function as a booster of chromosome condensation.Scientific Reports, 6: 38281.
doi: 10.1038/srep38281.

Nakahata Y, Nabekura J, and Murakoshi H. (2016) Dual observation of the ATP-evoked small GTPase activation and Ca2+ transient in astrocytes using a dark red fluorescent protein.Scientific Reports, 6: 39564.
doi: 10.1038/srep39564.

Murakoshi H, Yasuda R. (2016) Imaging signal transduction in dendrites using genetically-encoded fluorescent proteins. Dendritets: development and disease. Springer-Verlag, Germany. 139-154.

Murakoshi H., Yasuda R. (2016) Imaging signal transduction in dendrites using genetically-encoded fluorescent proteins. Dendritets: development and disease. Springer Japan, Germany. 139-154.
doi:10.1007/978-4-431-56050-0_7.

柴田明裕、村越秀治 (2016) 細胞内発現パターンを劇的に改善した高精度FRETセンサーの開発. 生物物理, 56-3: 181-184.

Imaging the activity of signaling molecules in deep tissue using "non-fluorescent" fluorescent proteins

2017

2016