Graduate School of Medicine, The University of Tokyo

Development of a probe-tag system for practical real-time nanoscopy

In this study, we will develop novel functional dyes and tag domains for the purpose of developing single-molecule localization microscopy with the principle of fluorescence blinking associated with binding and dissociation of dye and tag molecules.

The optimization of dye-tag domain systems will lead to the achievement of high temporal resolution in imaging and the development of a practical super-resolution imaging technique that will allow for visualization of nano-scale dynamics of target molecules in living cells.