In this study, we will develop novel functional dyes and tag domains for the purpose of developing single-molecule localization microscopy with the principle of fluorescence blinking associated with binding and dissociation of dye and tag molecules.
The optimization of dye-tag domain systems will lead to the achievement of high temporal resolution in imaging and the development of a practical super-resolution imaging technique that will allow for visualization of nano-scale dynamics of target molecules in living cells.
2018
Sakamoto H., Ariyoshi T., Kimpara N., Sugao K., Taiko I., Takikawa K., Asanuma D., Namiki S., Hirose K. (2018) Synaptic weight set by Munc13-1 supramolecular assemblies. Nature Neuroscience, 21: 41-49.
doi: 10.1038/s41593-017-0041-9.
2017
浅沼大祐、瀧川健司、並木繁行、廣瀬謙造 (2017) 神経伝達物質を高精細に可視化するハイブリッド型蛍光プローブの開発, 生体の科学, 公益財団法人金原一郎記念医学医療振興財団, 68 (5): 422-423.
doi: 10.11477/mf.2425200675.
2016
Hayashi A., Asanuma D., Kamiya M., Urano Y., Okabe S. (2016) High affinity receptor labeling based on basic leucine zipper domain peptides conjugated with pH-sensitive fluorescent dye: visualization of AMPA type glutamate receptor endocytosis in living neurons. Neuropharmacology, 100: 66-75.
doi:10.1016/j.neuropharm.2015.07.026.
Kitakaze K., Mizutani Y., Sugiyama E., Tasaki C., Tsuji D., Maita N., Hirokawa T., Asanuma D., Kamiya M., Sato K., Setou M., Urano Y., Togawa T., Otaka A., Sakuraba H., Itoh K. (2016) Protease-resistant modified human β-hexosaminidase B ameliorates symptoms in GM2 gangliosidosis model. J. Clin. Invest., 126: 1691-1703.
doi:10.1172/JCI85300.
Matsuzaki H., Kamiya M., Iwatate R. J., Asanuma D., Watanabe T., Urano Y. (2016) Novel Hexosaminidase-Targeting Fluorescence Probe for Visualizing Human Colorectal Cancer. Bioconjugate Chem., 27: 973–981.
doi:10.1021/acs.bioconjchem.6b00037.
