In this study, we will develop novel functional dyes and tag domains for the purpose of developing single-molecule localization microscopy with the principle of fluorescence blinking associated with binding and dissociation of dye and tag molecules.
The optimization of dye-tag domain systems will lead to the achievement of high temporal resolution in imaging and the development of a practical super-resolution imaging technique that will allow for visualization of nano-scale dynamics of target molecules in living cells.
2017
Sakamoto H., Ariyoshi T., Kimpara N., Sugao K., Taiko I., Takikawa K., Asanuma D., Namiki S. Hirose K. (2017) Synaptic weight set by Munc13-1 supramolecular assemblies. Nat. Neurosci., 21, 41-49.
doi:10.1038/s41593-017-0041-9.
2016
Hayashi A., Asanuma D., Kamiya M., Urano Y., Okabe S. (2016) High affinity receptor labeling based on basic leucine zipper domain peptides conjugated with pH-sensitive fluorescent dye: visualization of AMPA type glutamate receptor endocytosis in living neurons. Neuropharmacology, 100: 66-75.
doi:10.1016/j.neuropharm.2015.07.026.
Kitakaze K., Mizutani Y., Sugiyama E., Tasaki C., Tsuji D., Maita N., Hirokawa T., Asanuma D., Kamiya M., Sato K., Setou M., Urano Y., Togawa T., Otaka A., Sakuraba H., Itoh K. (2016) Protease-resistant modified human β-hexosaminidase B ameliorates symptoms in GM2 gangliosidosis model. J. Clin. Invest., 126: 1691-1703.
doi:10.1172/JCI85300.
Matsuzaki H., Kamiya M., Iwatate R. J., Asanuma D., Watanabe T., Urano Y. (2016) Novel Hexosaminidase-Targeting Fluorescence Probe for Visualizing Human Colorectal Cancer. Bioconjugate Chem., 27: 973–981.
doi:10.1021/acs.bioconjchem.6b00037.
