The development and function of living tissues are controlled by a diverse array of cell interactions that involve various extracellular messengers, such as neurotransmitters, hormones, autacoids, and growth factors. We have developed the Competitive Fluorescent Ligand Assay (CFLA), which can be applied to visualize these molecules. By applying this technique, we will develop fluorescent sensors that will allow for the visualization of acetylcholine, angiotensin II, growth factors, etc. In addition, we aim to elucidate the mechanisms of intercellular communication and the generation and regulation of biological rhythms by adding a mathematical model analysis, based on live imaging data on the vibration and synchronization of intracellular and extracellular messengers.
2019
Nezu A., Morita T., Nagai T., Tanimura A. (2019) Simultaneous monitoring of Ca2+ responses and salivary secretion in live animals reveals a threshold intracellular Ca2+ concentration for salivation. Exp. Ohysiol., 104(1):61-69.
doi: 10.1113/EP086868.
2018
谷村明彦, 根津顕弘, 森田貴雄, 村田佳織. (2018) IP3シグナル解析法の進歩と細胞内シグナル研究への応用. 日本薬理学雑誌, 152 (1): 21-27.
Tanimura A. (2018) InVivo imaging study for the regulation of salivary gland functions. Impact, 2018 (2): 38-40.
doi:10.21820/23987073.2018.2.38.
2017
谷村明彦. (2017) IP3センサー. 生体の科学, 68 (5): 448-449.
doi: 10.11477/mf.2425200688.
Sneyd J., Han J. M., Wang L., Chen J., Yang X., Tanimura A., Sanderson M. J., Kirk V. and Yule D. I. (2017) On the dynamical structure of calcium oscillations. Proc. Natl. Acad. Sci. USA., 114 (7): 1456-1461.
doi:10.1073/pnas.1614613114.
Han J. M., Tanimura A., Kirk V. and Sneyd J. (2017) A mathematical model of calcium dynamics in HSY cells. PLoS Comput. Biol., 13 (2): e1005275.
doi:10.1371/journal.pcbi.1005275.