Health Sciences University of Hokkaido, School of Dentistry

Development of fluorescent tethered ligand sensors and analyses of inter cellular communications

The development and function of living tissues are controlled by a diverse array of cell interactions that involve various extracellular messengers, such as neurotransmitters, hormones, autacoids, and growth factors. We have developed the Competitive Fluorescent Ligand Assay (CFLA), which can be applied to visualize these molecules. By applying this technique, we will develop fluorescent sensors that will allow for the visualization of acetylcholine, angiotensin II, growth factors, etc. In addition, we aim to elucidate the mechanisms of intercellular communication and the generation and regulation of biological rhythms by adding a mathematical model analysis, based on live imaging data on the vibration and synchronization of intracellular and extracellular messengers.